Recent Publications


A complete collection of Josh Leonard’s publications can be found at Pubmed or Google Scholar.


Nathamgari SSP, Pathak N, Lemaitre V, Mukherjee P, Muldoon JJ, Peng C, McGuire T, Leonard JN, Kessler JA, Espinosa HD (2020). Nano Fountain Probe Electroporation Enables Versatile Single-Cell Intracellular Delivery and Investigation of Post-Pulse Electro-pore Dynamics. Small. In press.

Edelstein HI, Donahue PS, Muldoon JJ, Kang AK, Dolberg TB, Battaglia LM, Allchin ER, Hong M, and Leonard JN (2020). Elucidation and refinement of synthetic receptor mechanisms. bioRxiv. e-pub.



Muldoon JJ, Chuang Y, Bagheri N, and Leonard JN (2020). Macrophages employ quorum licensing to regulate collective activation. Nature Communications. e-pub.





Donahue PS, *Draut JW, *Muldoon JJ, *Edelstein HI, Bagheri N, and Leonard JN (2020). The COMET toolkit for composing customizable genetic programs in mammalian cells. Nature Communications. *co-second authors.  e-pub.


*Dolberg TB, *Meger AT, Boucher JD, Corcoran WK, Schauer EE, Prybutok AN, Raman S, and Leonard JN (2019). Computation-guided optimization of split protein systems. bioRxiv. *co-first authors. e-pub.


Kang Li, Rodosthenous RS, Kashanchi F, Gingeras T, Gould SJ, Kuo LS, Kurre P, Lee H, Leonard JN, Liu H, Lombo TB, Momma S, Nolan JP, Ochocinska MJ, Pegtel DM, Sadovsky Y,  Sánchez-Madrid F, Valdes KM, Vickers KC, Weaver AM, Witwer KW, Zeng Y, Das S, Raffai RL, and Howcroft TK (2018). Advances, challenges, and opportunities in extracellular RNA biology: insights from the NIH exRNA Strategic Workshop. JCI Insight. e-pub.


Dolberg TB, Donahue PS, Leonard JN (2018). Synthetic biology: Reframing cell therapy for cancer. Nature Chemical Biology. e-pub


Hung ME, Lenzini SB, Stranford DM, Leonard JN (2018). Enrichment of Extracellular Vesicle Subpopulations Via Affinity Chromatography. Methods in Molecular Biology. Pubmed

Hong BJ, Iscen A, Chipre A, Li MM, Lee OS, Leonard JN, Schatz G, Nguyen SB (2018). Highly Stable, Ultra-Small Polymer-Grafted Nanobins (usPGNs) with Stimuli-Responsive Capability. Journal of Physical Chemistry. Pubmed


Younger AKD, Su PY, Shepard AJ, Udani SV, Cybulski TR, Tyo KE, Leonard JN (2018). Development of novel metabolite-responsive transcription factors via transposon-mediated protein fusion. Protein Engineering, Design, and Selection. Pubmed


Nut C, Rodriguez E, Donahue PS, Stranford DM, Leonard JN (2017). An investigation of petty theft of beach decorations at American universities. Journal Criminal Justice. Read more

Muldoon JJ, Donahue PS, Dolberg TB, Leonard JN (2017). Building with intent: Technologies and principles for engineering mammalian cell-based therapies to sense and respond. Current Opinion in Biomedical Engineering. Epub ahead of print

Xiao X, Wang Y, Leonard JN, Hall CK (2017). Extended Concerted Rotation Technique Enhances the Sampling Efficiency of the Computational Peptide-Design Algorithm. Journal of Chemical Theory and Computation. Pubmed


Stranford DM, Leonard JN (2017). Delivery of Biomolecules via Extracellular Vesicles: A Budding Therapeutic Strategy. Advances in Genetics. Pubmed

Hartfield RM, Schwarz KA, Muldoon JJ, Bagheri N, Leonard JN (2017). Multiplexing engineered receptors for multiparametric evaluation of environmental ligands. ACS Synthetic Biology. Pubmed

Stranford DM, Hung ME, Gargus ES, Shah RN, Leonard JN (2017). A systematic evaluation of factors affecting extracellular vesicle uptake by breast cancer cells. Tissue Engineering Part A. Pubmed (Invited contribution to special issue)


leonard-graphical-abstract-01Schwarz KA, Daringer NM, Dolberg TB, Leonard JN (2017). Rewiring human cellular input–output using modular extracellular sensors. Nature Chemical Biology. Pubmed



abstract-graphicYounger AKD, Dalvie NC, Rottinghaus AG, Leonard JN (2016). Engineering modular biosensors to confer metabolite-responsive regulation of transcription. ACS Synthetic Biology. Pubmed


abstractgraphic-01Du Lac M, Scarpelli AH, Younger AKD, Bates DG, Leonard JN (2016). Predicting the dynamics and heterogeneity of genomic DNA content within bacterial populations across variable growth regimes. ACS Synthetic Biology. Pubmed


Chuang_II_Fig4Chuang YC, Hung ME, Cangelose BK, Leonard JN (2016). Regulation of the IL-10-driven macrophage phenotype under incoherent stimuli. Innate Immunity. Pubmed


hungXaio-and-HungXaio X, Hung ME, Leonard JN, Hall CK (2016). Adding energy minimization strategy to peptide-design algorithm enables better search for RNA-binding peptides: Redesigned λ N peptide binds boxB RNA. Journal of Computational Chemistry. Pubmed



schwarz-reveiwAbstractFigureSchwarz KA, Leonard JN. (2016) Engineering cell-based therapies to interface robustly with host physiology. Advanced Drug Delivery Reviews. Pubmed


Figure 1 16-0316Hung ME, Leonard JN (2016). A platform for actively loading cargo RNA to elucidate limiting steps in EV-mediated delivery. Journal Extracellular Vesicles. Pubmed


gowerGower 2016Liu JM, Zhang J, Zhang X, Hlavaty KA, Ricci CF, Leonard JN, Shea LD, Gower RM. (2016) Transforming growth factor-beta 1 delivery from microporous scaffolds decreases inflammation post-implant and enhances function of transplanted islets. Biomaterials. Pubmed

Other Publications


Jelinek I, Leonard JN, Price G, Brown K, Meyer-Manlapat A, Goldsmith PK, Wang Y, Venzon D, Epstein SL, Segal DM. TLR3-specific dsRNA oligonucleotide adjuvants induce dendritic cell cross-presentation, CTL responses, and antiviral protection. Journal of Immunology. 2011 186 (4): 2422-9. Pubmed

Leonard JN, Bell JK, Segal DM. Predicting TLR structures and characterizing ligand binding. Methods in Molecular Biology. 2009 517:55-67. Pubmed

Leonard JN, Ghirlando R, Askins J, Bell JK, Margulies DH, Davies DR, Segal DM. The TLR3 signaling complex forms by cooperative receptor dimerization. Proceedings of the National Academy of Sciences USA. 2008 105(1):258-63. Pubmed

Liu L, Botos I, Wang Y, Leonard JN, Shiloach J, Segal DM, Davies DR. Structural basis of toll-like receptor 3 signaling with double-stranded RNA. Science. 2008 320(5874):379-81. Pubmed

Leonard JN*, Shah PS*, Burnett JC, Schaffer DV. HIV evades RNA interference directed at TAR by an indirect compensatory mechanism. Cell Host & Microbe. 2008 4(5):484-94. *co-first authors. Pubmed

Ricci C, Pastukh V, Leonard JN, Turrens J, Wilson G, Schaffer DV, Schaffer SW. Mitochondrial DNA damage triggers mitochondrial-superoxide generation and apoptosis. American Journal of Physiology. 2008 294, C413-22. Pubmed

Zhang SY, Jouanguy E, Ugolini S, Smahi A, Elain G, Romero P, Segal D, Sancho-Shimizu V, Lorenzo L, Puel A, Picard C, Chapgier A, Plancoulaine S, Titeux M, Cognet C, von Bernuth H, Ku CL, Casrouge A, Zhang XX, Barreiro L, Leonard J, Hamilton C, Lebon P, Héron B, Vallée L, Quintana-Murci L, Hovnanian A, Rozenberg F, Vivier E, Geissmann F, Tardieu M, Abel L, Casanova JL. TLR3 deficiency in patients with herpes simplex encephalitis. Science. 2007 317:1522-7. Pubmed

Ashton RS, Peltier J, Fasano CA, O’neill A, Leonard J, Temple S, Schaffer DV, Kane R. High-throughput screening of gene function in stem cells using clonal microarrays. Stem Cells. 2007 25:2928-35. Pubmed

Leonard JN, Ferstl P, Delgado A, Schaffer DV. Enhanced preparation of adeno-associated viral vectors by using high hydrostatic pressure to selectively inactivate helper adenovirus. Biotechnology and Bioengineering. 2007  97:1170-9. (Featured in a spotlight in this issue) Pubmed

Leonard JN, Schaffer DV. Antiviral RNAi therapy: emerging approaches for hitting a moving target. Gene Therapy. 2006 13(6):532-40. Pubmed

Leonard JN, Schaffer DV. Computational design of antiviral RNA interference strategies that resist human immunodeficiency virus escape. Journal of Virology. 2005 79(3):1645-54. Pubmed



Leonard JN, Dudek RM, Daringer NM, inventors; 2012 Aug 3. Modular Extracellular Sensor Architecture for Cell-based Biosensors. United States Patent 9,732,392. Download

Schaffer DV, Leonard JN, inventors; 2010 Dec. 14. Methods for Purifying Adeno-Associated Virus Particles. United States Patent 7,851,196. Download

Schaffer DV, Leonard JN, inventors; 2009 Dec. 1. Methods for Purifying Adeno-Associated Virus. United States Patent 7,625,570. Download

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