Recent publications

A complete collection of Josh Leonard’s publications can be found at Pubmed or Google Scholar.

Schwarz KA, Daringer NM, Dolberg TB, Leonard JN (2017) Rewiring human cellular input–output using modular extracellular sensors. Nature Chemical Biology. Pubmed



Younger AKD, Dalvie NC, Rottinghaus AG, Leonard JN (2016) Engineering modular biosensors to confer metabolite-responsive regulation of transcription. ACS Synthetic Biology. Pubmed


Du Lac M, Scarpelli AH, Younger AKD, Bates DG, Leonard JN (2016) Predicting the dynamics and heterogeneity of genomic DNA content within bacterial populations across variable growth regimes. ACS Synthetic Biology. Pubmed


Chuang YC, Hung ME, Cangelose BK, Leonard JN (2016) Regulation of the IL-10-driven macrophage phenotype under incoherent stimuli. Innate Immunity. epub ahead of press.


Xaio X, Hung ME, Leonard JN, Hall CK. (2016) Adding energy minimization strategy to peptide-design algorithm enables better search for RNA-binding peptides: Redesigned λ N peptide binds boxB RNA. J Comput Chem. Pubmed


Schwarz KA, Leonard JN. (2016) Engineering cell-based therapies to interface robustly with host physiology. Advanced Drug Delivery Reviews. Pubmed


Hung ME, Leonard JN. (2016) A platform for actively loading cargo RNA to elucidate limiting steps in EV-mediated delivery. J Extracell Vesicles. Pubmed

Figure 1 16-0316


Liu JM, Zhang J, Zhang X, Hlavaty KA, Ricci CF, Leonard JN, Shea LD, Gower RM. (2016) Transforming growth factor-beta 1 delivery from microporous scaffolds decreases inflammation post-implant and enhances function of transplanted islets. Biomaterials. Pubmed

Gower 2016gower

Other Publications


Jelinek I, Leonard JN, Price G, Brown K, Meyer-Manlapat A, Goldsmith PK, Wang Y, Venzon D, Epstein SL, Segal DM. TLR3-specific dsRNA oligonucleotide adjuvants induce dendritic cell cross-presentation, CTL responses, and antiviral protection. Journal of Immunology. 2011 186 (4): 2422-9. Pubmed


Leonard JN, Bell JK, Segal DM. Predicting TLR structures and characterizing ligand binding. Methods in Molecular Biology. 2009 517:55-67. Pubmed


Leonard JN, Ghirlando R, Askins J, Bell JK, Margulies DH, Davies DR, Segal DM. The TLR3 signaling complex forms by cooperative receptor dimerization. Proc Natl Acad Sci USA. 2008 105(1):258-63. Pubmed


Liu L, Botos I, Wang Y, Leonard JN, Shiloach J, Segal DM, Davies DR. Structural basis of toll-like receptor 3 signaling with double-stranded RNA. Science. 2008 320(5874):379-81. Pubmed


Leonard JN*, Shah PS*, Burnett JC, Schaffer DV. HIV evades RNA interference directed at TAR by an indirect compensatory mechanism. Cell Host Microbe. 2008 4(5):484-94. *co-first authors. Pubmed


Ricci C, Pastukh V, Leonard JN, Turrens J, Wilson G, Schaffer DV, Schaffer SW. Mitochondrial DNA damage triggers mitochondrial-superoxide generation and apoptosis. American Journal of Physiology. 2008 294, C413-22. Pubmed


Zhang SY, Jouanguy E, Ugolini S, Smahi A, Elain G, Romero P, Segal D, Sancho-Shimizu V, Lorenzo L, Puel A, Picard C, Chapgier A, Plancoulaine S, Titeux M, Cognet C, von Bernuth H, Ku CL, Casrouge A, Zhang XX, Barreiro L, Leonard J, Hamilton C, Lebon P, Héron B, Vallée L, Quintana-Murci L, Hovnanian A, Rozenberg F, Vivier E, Geissmann F, Tardieu M, Abel L, Casanova JL. TLR3 deficiency in patients with herpes simplex encephalitis. Science. 2007 317:1522-7. Pubmed


Ashton RS, Peltier J, Fasano CA, O’neill A, Leonard J, Temple S, Schaffer DV, Kane R. High-throughput screening of gene function in stem cells using clonal microarrays. Stem Cells. 2007 25:2928-35. Pubmed


Leonard JN, Ferstl P, Delgado A, Schaffer DV. Enhanced preparation of adeno-associated viral vectors by using high hydrostatic pressure to selectively inactivate helper adenovirus. Biotechnology and Bioengineering. 2007  97:1170-9. (Featured in a spotlight in this issue) Pubmed


Leonard JN, Schaffer DV. Antiviral RNAi therapy: emerging approaches for hitting a moving target. Gene Therapy. 2006 13(6):532-40. Pubmed


Leonard JN, Schaffer DV. Computational design of antiviral RNA interference strategies that resist human immunodeficiency virus escape. Journal of Virology. 2005 79(3):1645-54. Pubmed



Leonard JN, Dudek RM, Daringer NM, inventors; 2012 Aug 3. Modular Extracellular Sensor Architecture for Cell-based Biosensors. United States Patent (pending).


Schaffer DV, Leonard JN, inventors; 2010 Dec. 14. Methods for Purifying Adeno-Associated Virus Particles. United States Patent 7,851,196. Download


Schaffer DV, Leonard JN, inventors; 2009 Dec. 1. Methods for Purifying Adeno-Associated Virus. United States Patent 7,625,570. Download


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